Life Science Automation Fundamentals and Applications.pdf

Life Science Automation

Fundamentals and Applications

For a list of related Artech House titles,

please see the back of this book.

Life Science Automation

Fundamentals and Applications

Mingjun Zhang

Bradley Nelson

Robin Felder

Editors

Library of Congress Cataloging-in-Publication Data

A catalog record for this book is available from the U.S. Library of Congress.

British Library Cataloguing in Publication Data

A catalogue record for this book is available from the British Library.

Cover design by Igor Valdman

685 Canton Street

Norwood, MA 02062

ISBN-13: 978-1-59693-105-3

may be reproduced or utilized in any form or by any means, electronic or mechanical, includ-

ing photocopying, recording, or by any information storage and retrieval system, without

permission in writing from the publisher.

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been appropriately capitalized. Artech House cannot attest to the accuracy of this informa-

tion. Use of a term in this book should not be regarded as affecting the validity of any trade-

mark or service mark.

10 9 8 7 6 5 4 3 2 1

Contents

Preface

PART I

Life Science Basis for Automation

CHAPTER 1

Introduction to Nucleic Acids and Clinical Laboratory Testing 3

1.1 Basics of Nucleic Acid Structure 4

1.1.1 General Principles 4

1.1.2 In Vitro Aspects of DNA 5

1.1.3 Unidirectional Flow of Genetic Information: The Central Dogma 9

1.2 Manipulation of DNA Under Testing Conditions

1.2.1 Extraction

1.2.2 Amplification

1.2.3 Detection

1.3 Statistics and Test Utilization Used in Medical Decision Making

1.3.1 Sensitivity and Specificity

1.3.2 Predictive Values

1.3.3 Preanalytical Versus Analytical Stages of Testing

1.4 Summary

Reference

CHAPTER 2

Basic Analytical Chemistry for Engineers

2.1 Introduction

2.2 Chromatographic Separation Methods

2.2.1 General Principles

2.2.2 Gas Chromatographic Methods

2.2.3 Liquid Chromatographic Methods

2.2.4 Electrophoresis

2.3 Bioanalytical Detection Methods

2.3.1 Protein Analysis

2.3.2 DNA and RNA Analysis

2.3.3 Enzymatic Analysis

2.3.4 Immunological Methods

2.4 Physical Detection Methods

2.4.1 Atomic Spectroscopy

2.4.2 Optical Molecule Spectroscopy

2.4.3 Nuclear Magnetic Resonance Spectroscopy

2.4.4 Mass Spectrometry

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